https://doi.org/10.36719/2663-4619/112/105-110
Fatma Huseynova
Scientific Research Institute of Medical Prevention after the name of V.Y.Akhundov
PhD in medical sciences
https://orcid.org/0009-0009-6805-2302
huseynova01049@gmail.com
Shahla Aliyeva
Scientific Research Institute of Medical Prevention after the name of V.Y.Akhundov
PhD in biological sciences
https://orcid.org/0009-0006-6971-4679
shahlaaliyeva1969@gmail.com
Zivar Abseynova
Scientific Research Institute of Medical Prevention after the name of V.Y.Akhundov
https://orcid.org/0009-0009-1366-0896
ziver.abseynova@mail.ru
Ilhame Masimova
Scientific Research Institute of Medical Prevention after the name of V.Y.Akhundov
https://orcid.org/0009-0004-5507-3650
ilhamemasimova73@gmail.com
Sevda Rahimli
Scientific Research Institute of Medical Prevention after the name of V.Y.Akhundov
https://orcid.org/0009-0001-0332-9667
sevda.rahimli90@gmail.com
The Role of Physical Factors in the Cultivation of Plasmodium Vivax in Vitro
Abstract
P.vivax spreads over a wide geographic area, infecting up to 80 million people each year. The absence of a long-term culture of the pathogen in vitro makes it difficult to effectively control this type of malaria and create a vaccine. The complex biological characteristics of P.vivax prevent its long-term culture. There is currently no standard protocol for the continuous cultivation of this plasmodium species. One of the reasons for not obtaining continuous culture of P.vivax is the inability of merozoites to enter the host cell. According to the researchers, many aspects should be considered in order to standardize the culture of P.vivax.
This article discusses various physical factors that are important for obtaining in vitro a continuous culture of the causative agent of malaria tertiana (Plasmodium vivax). Proper choice of parasite source, blood sample storage conditions, static or dynamic culture system, temperature are some of the factors that may be important for continuous culture of P.vivax in vitro. Another question concerns the selection of static or dynamic culture conditions for in vitro development of the parasite. Some researchers have alternately used these 2 culture systems for P.vivax.
Keywords: malaria, P.vivax, continuous culture, source of parasites, isolate, static and dynamic culture