https://doi.org/10.36719/2789-6919/42/144-150
Khadija Rzazadeh
Azerbaijan Medical University
student
https://orcid.org/0009-0000-5160-1605
xadicar@mail.ru
Ultrastructural Features of the Effect of Systemic İnflammation on Cardiomyocytes
Abstract
This article discusses the changes caused by the action of lipopolysaccharide (endotoxin) on the myocardium. LPS induces intracellular transcription of a number of inflammatory mediators by interacting with receptors on many tissue cells, cells of the innate immune system, skeletal muscle cells and adipocytes. Endotoxin also leads to the progression of atherosclerosis, heart failure and serious changes in the myocardium. The conclusions made in the presented article were drawn up on the basis of the experiment. Experimental endotoxemia was caused by intraperitoneal administration of e.coli lipopolysaccharide to mice. Myocardial sections of the control and experimental groups were fixed in solution. Araldite-Epon blocks were prepared according to general techniques adopted in electron microscopy. The histological preparations obtained showed signs of inflammation (edema) among myocardial cells, infiltration of the myocardium with polynuclear neutrophilic leukocytes, as well as an abundance of monocytes and macrophages. Electron microscopy in cardiomyocytes showed a significant expansion of the T-tubules formed by their plasmalemma. In addition, mitochondrial damage was noted. From the results obtained and the studies conducted, it is clear that the factors leading to impaired cardiac function in sepsis include edema of the heart myocardium, infiltration of its interstitial tissue with immune cells, and damage to mitochondria in cardiomyocytes. The article includes ideas about changes and disorders in the myocardium in the form of drawings of histological preparations.
Keywords: lipopolysaccharide, endotoxin, myocardium, cardiomyocytes, mitochondria, myofibrils, T-tubules